Journal: Nucleic Acids Research
Article Title: Increased in vivo inhibition of gene expression by combining RNA interference and U1 inhibition
doi: 10.1093/nar/gkr956
Figure Lengend Snippet: Schematic of U1i and inhibition of Notch1 in vivo . ( a ) Schematic of U1-mediated inhibition. U1in are exogenous 5′-end modified U1 snRNPs designed to base pair to the 3′-terminal exon of a target gene. In this study, the U1in binds Notch1 or HBV mRNA and blocks expression by hindering polyadenylation (pA). ( b ) C57BL/6 mice were injected with pNFκβ-Luc plasmid (NFkBLuc) combined or not with plasmids expressing shαNotch1 (sh), U1inαNotch1 (U1in) or the combination of both. Luciferase activity was measured in living mice with a CCD camera at 4 days post-injection. Luciferase activity was quantified and plotted. The SI and the statistical significance are indicated.
Article Snippet: The pCH Firefly Luc vector (pCH-Fluc) was constructed by replacing the preS2/S ORF region of pCH-9/3091 HBV replication competent plasmid with Firefly luciferase-encoding DNA ( ). pNFκβ-Luc (pNFκβ 3xLuc; Clontech Co) was used to express Firefly luciferase under pNFκβ promoter.
Techniques: Inhibition, In Vivo, Modification, Expressing, Injection, Plasmid Preparation, Luciferase, Activity Assay